Characterization, mRNA expression profile, subcellular distribution and association analysis with piglet diarrhea of porcine matrix metallopeptidase 7 (pMMP7)

• Published in: Gene Vol. 821; p. 146319
• Main Authors: Niu, Buyue, Liu, Lu, Chen, Zhihua, Kou, Mingxing, Yang, Xiuqin, Sun, Yuan, Di, Shengwei, Wang, Xibiao, Cai, Jiancheng, Guo, Dongchun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.05.2022
• Subjects: Animals; Breeding; Cytoplasm - metabolism; Diarrhea - genetics; Diarrhea - metabolism; Diarrhea - veterinary; Gene Expression Profiling; Gene Expression Regulation, Enzymologic; Genetic Association Studies; Genetic variations; Genotype; Matrix Metalloproteinase 7 - genetics; Matrix Metalloproteinase 7 - metabolism; MMP7; mRNA Expression; Polymorphism, Single Nucleotide; Porcine; Subcellular distribution; Sus scrofa - genetics; Sus scrofa - metabolism; Swine; Swine Diseases - genetics; Swine Diseases - metabolism; Tissue Distribution; Porcine; mRNA Expression; ORF; MMP7; TIMP-1; ADG; MMPs; IBD; ECM; CXCL11; UTR; Genetic variations; CDS; SNP; SPP1; pMMP7; cDNA; qPCR; CD14; Subcellular distribution; CD44; PCR
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2022.146319

•The complete coding sequence of pMMP7 is 804 bp.•pMMP7 mRNA expression in the intestine of healthy SPF piglets were weak.•pMMP7-EGFP fusion protein located mainly outside the nucleus.•The diarrhea score of TT or AA Landrace piglet was low. Matrix metalloproteinase 7 (MMP7) is involved in the degradation of extracellular matrix in disease processes and therefore plays an important role in host disease resistance/susceptibility. To better understanding the effects of porcine MMP7 (pMMP7) on piglets diarrhea trait, we characterized pMMP7 gene, identified genetic variations in pMMP7 and explored the relationship between pMMP7 polymorphisms and piglets diarrhea in Min pig and Landrace populations. The complete coding sequence of pMMP7 is 804 bp encoding a protein of 267 amino acids. Sequence alignment showed that the identity between pMMP7 and human MMP7 was approximately 80%. The expression of pMMP7 in the gut of healthy piglets were weak and the distribution of the pMMP7-EGFP fusion protein was observed mainly in the cytoplasm. After the identification of 21 genetic variations in 5′ flanking region and exons, Hae III and Eco72 Ⅰ PCR-RFLP were established to genotype SNP rs327380117 and rs329429922, respectively. Statistical analysis indicated that Landrace piglets with a TT genotype at rs327380117 had a lower diarrhea score and day-14 wt than TC piglets (p < 0.05); the diarrhea score of AA Landrace animals with rs329429922 was lower than that of GG individuals (p < 0.05). The findings presented here contribute to the understanding of the biological function of pMMP7 and may provide new molecular markers for pig breeding.