Chemical and bioactivity screening of subcritical water extracts of chokeberry (Aronia melanocarpa) stems

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 164; pp. 353 - 359
• Main Authors: Švarc-Gajić, Jaroslava, Cerdà, Víctor, Clavijo, Sabrina, Suárez, Ruth, Zengin, Gökhan, Cvetanović, Aleksandra
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.02.2019
• Subjects: Anti-diabetic activity; Chokeberry (Aronia melanocarpa) stems; GC–MS; Subcritical water extraction; Tyrosinase inhibition; Tyrosinase inhibition; GC–MS; Anti-diabetic activity; Chokeberry (Aronia melanocarpa) stems; Subcritical water extraction
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2018.11.006

•Aronia stems were extracted by subcritical water.•Chemical profile was defined by GC–MS.•Antioxidant potential was determined by DPPH test and Reducing Power test.•Anti-diabetic activity was defined by glucosidase and amylase inhibition potential.•Tyrosinase-inhibitory potential of extracts was defined. Subcritical water extracts of chokeberry (Aronia melanocarpa) stems were chemically and biologically characterised. Chemical profile was defined by GC–MS analysis whereas anti-oxidant, anti-diabetic and tyrosinase-inhibitory activities of the extracts were investigated by in vitro assays. Antioxidant activity assays revealed strong activity against DPPH radical (IC50 = 0.1 mg/mL) and reducing power (IC50 = 0.25 mg/mL). The extracts demonstrated remarkable amylase (0.59 mmol ACAE/g) and glucosidase (7.50 mmol ACAE/g) inhibitory effects. Anti-tyrosinase activity of aronia stem extracts obtained by subcritical water was calculated to be 15.87 mg KAE/g extract. GC–MS analysis of chokeberry stem subcritical water extracts revealed the presence of different chemical classes. The compounds present in the highest concentrations were polyols arabitol (13.7%), xylitol (3.5%), and glycerol (1.96%), as well as sugars such as fructose (3.04%), ribose (1.99%) and xylulose (1.18%).