Morphology, morphogenesis and molecular phylogeny of a freshwater ciliate, Monomicrocaryon euglenivorum euglenivorum (Ciliophora, Oxytrichidae)

• Published in: European journal of protistology Vol. 68; pp. 25 - 36
• Main Authors: Shao, Chen, Hu, Chunxu, Fan, Yangbo, Warren, Alan, Lin, Xiaofeng
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.04.2019
• Subjects: Hypotrichia; Molecular systematics; Ontogenesis; SSU rDNA; Taxonomy; Ontogenesis; SSU rDNA; Molecular systematics; Hypotrichia; Taxonomy
• ISSN: 0932-4739; 1618-0429
• DOI: 10.1016/j.ejop.2019.01.001

The morphology, morphogenesis and molecular phylogeny of the oxytrichid ciliate, Monomicrocaryon euglenivorum euglenivorum (Kahl, 1932) Foissner, 2016, isolated from freshwater in a seaside park, Guangzhou, China, were investigated. Monomicrocaryon euglenivorum euglenivorum can be recognized as follows: caudal cirri in midline of body; dorsal kinety 1 without a one-kinetid-wide gap; transverse cirri acicular or rod-shaped with a fringed distal end; right marginal row commences at level of buccal vertex or anterior to buccal vertex. The main events during binary fission are as follows: (1) the proter retains the parental adoral zone of membranelles entirely; (2) frontoventral-transverse cirral anlagen I–VI are segmented in the ordinary pattern 1:3:3:3:4:4 from left to right, which form three frontal, four frontoventral, one buccal, three postoral ventral, two pretransverse ventral and five transverse cirri, respectively; (3) dorsal morphogenesis is in the typical Oxytricha-pattern, but fragmentation of dorsal kinety 3 is indistinct; and (4) three caudal cirri are formed, one at the posterior end of each of dorsal kineties 1, 2 and 4. Phylogenetic analyses based on SSU rDNA sequences showed that M. euglenivorum euglenivorum clustered with Kleinstyla dorsicirrata and Heterourosomoida lanceolata rather than with its congener M. elegans. The genus Monomicrocaryon is not monophyletic in this study; however, its monophyly is not rejected by the AU test.