A radioimmunoassay for the estimation of serum dehydroepiandrosterone sulphate in normal and pathological sera

• Published in: Clinica chimica acta Vol. 65; no. 1; pp. 5 - 13
• Main Authors: Smith, Margaret R., Rudd, B.T., Shirley, Anne, Rayner, P.H.W., Williams, J.W., Duignan, N.M., Bertrand, P.V.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.11.1975
• Subjects: Adolescent; Adult; Aged; Child; Child, Preschool; Dehydroepiandrosterone - blood; Dehydroepiandrosterone - immunology; Female; Humans; Infant; Infant, Newborn; Male; Microchemistry; Middle Aged; Pregnancy; Radioimmunoassay - methods; Steroid Hydroxylases - deficiency; Time Factors
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/0009-8981(75)90328-9

A radioimmunoassay is described for the measurement of dehydroepiandrosterone sulphate in serum. The method requires 0.1 ml of serum that needs no purification other than a single ether extraction. It is both rapid and economical, procedural losses are small and precision and accuracy are acceptable for both normal and pathological sera. Normal values have been established for children and adults which show a relationship to chronological age, values rising steadily as puberty intervenes, peak values occurring between 20 and 27 years, with a decline thereafter. An empirical curve was fitted to the data and approximate normal limits were obtained. The mean value for pregnant women in the age range 20–40 years, was statistically significantly lower than in non-pregnant, premenopausal women. The levels of dehydroepiandrosterone sulphate in cord and newborn blood, were higher than at any other time during childhood until signs of puberty were demonstrable. Elevated levels are reported in six patients with untreated congenital adrenal hyperplasia and in one patient with a tumour of the adrenal cortex. It is suggested that this assay provides a useful and precise index of adrenal androgen secretion.