A simple solid-phase amino acid sequencer employing a thioacetylation stepwise degradation procedure

A simple inexpensive solid-phase amino acid sequencing device has been constructed which uses nitrogen pressure for introducing or removing reagents to or from a reaction vessel. Although a thioacetylation procedure was employed in the operations reported here, any stepwise degradation scheme can be...

Full description

Saved in:
Bibliographic Details
Published inAnalytical biochemistry Vol. 78; no. 2; pp. 491 - 505
Main Authors Doolittle, L.R., Mross, G.A., Fothergill, L.A., Doolittle, R.F.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.04.1977
Subjects
Acetylation
Amino Acids - analysis
Autoanalysis
Chemical Phenomena
Chemistry
Glass
Methods
Peptides
Online AccessGet full text

Cover

More Information
Summary:A simple inexpensive solid-phase amino acid sequencing device has been constructed which uses nitrogen pressure for introducing or removing reagents to or from a reaction vessel. Although a thioacetylation procedure was employed in the operations reported here, any stepwise degradation scheme can be used simply by changing the contents of the reservoirs. Flexible programming has been achieved with a Tenor stepping-drum programmer. The results obtained from a 26-cycle degradation of the insulin B-chain are presented.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(77)90109-9