Intracellular Trafficking Characteristics for Magnetic Layered Double Hydroxide/DNA Hybrids to Human Gastric Cancer Cells

We report here the intracellular trafficking characteristics of a gene delivery system composed by magnetic layered double hydroxide/DNA (MLDH/DNA) hybrids to human gastric cancer cells (SGC-7901). Perls Staining of MLDH/DNA was done to examine the intracellular iron uptake and accumulation; confoca...

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Bibliographic Details
Published inApplied Mechanics and Materials Vol. 320; pp. 529 - 532
Main Authors Dong, Li E, Sun, Yue, Gou, Guo Jing
Format Journal Article
LanguageEnglish
Published Zurich Trans Tech Publications Ltd 01.05.2013
Subjects
Intracellular Trafficking
Gene Delivery
Magnetic Layered Double Hydroxide/DNA Hybrids
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Summary:We report here the intracellular trafficking characteristics of a gene delivery system composed by magnetic layered double hydroxide/DNA (MLDH/DNA) hybrids to human gastric cancer cells (SGC-7901). Perls Staining of MLDH/DNA was done to examine the intracellular iron uptake and accumulation; confocal microscopy and transmission electron microscopy were evaluated the cellular internalization feature of the MLDH/DNA system. We have indicated that the MLDH/DNA hybrids are able to enter into the nucleus within 2 h of incubation, displaying a prospect for application in targeted gene delivery.
Bibliography:Selected, peer reviewed papers from the 2012 China Functional Materials Technology and Industry Forum (CFMTIF 2012), November 9-12, 2012, Kunming, China
ISBN:9783037856857
3037856858
ISSN:1660-9336
1662-7482
1662-7482
DOI:10.4028/www.scientific.net/AMM.320.529