Preformulation Studies with Phenylalanine Ammonia Lyase: Essential Prelude to a Microcapsule Formulation for the Management of Phenylketonuria

• Published in: Journal of pharmaceutical sciences Vol. 111; no. 7; pp. 1857 - 1867
• Main Authors: Besada, Christina, Hakami, Abrar, Pillai, Gayatri, Yetsko, Kyle, Truong, Nhu, Little, Timothy, Pantano, Sharon, Dmello, Anil
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2022
• Subjects: Albumin; Enzyme kinetics; Gastrointestinal tract; HPLC; Microencapsulation; Preformulation; Protein binding; Protein formulation; Stability; Km; Stability; Vmax; Albumin; g.i.t; Gastrointestinal tract; Phe; BH4; BSA; TCA; PAH; Preformulation; Microencapsulation; PAL; PKU; Enzyme kinetics; Protein formulation; HPLC; Protein binding

[Display omitted] . Phenylalanine ammonia lyase (PAL) metabolizes phenylalanine to transcinnamic acid (TCA). Our eventual goal is to develop a PAL microcapsule formulation to deplete phenylalanine in the gastrointestinal tract (g.i.t). The focus of this research is pre-formulation studies with PAL....