Inhibition of Organic Anion Transporting Polypeptide 1B1 and 1B3 by Betulinic Acid: Effects of Preincubation and Albumin in the Media

• Published in: Journal of pharmaceutical sciences Vol. 107; no. 6; pp. 1713 - 1723
• Main Authors: Oh, Yunseok, Jeong, Yoo-Seong, Kim, Min-Soo, Min, Jee Sun, Ryoo, Gongmi, Park, Ji Eun, Jun, Yearin, Song, Yoo-Kyung, Chun, Se-Eun, Han, Songhee, Bae, Soo Kyung, Chung, Suk-Jae, Lee, Wooin
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2018
• Subjects: Animals; Cattle; drug interactions; drug transport; food interactions; HEK293 Cells; Humans; Liver-Specific Organic Anion Transporter 1 - antagonists & inhibitors; Liver-Specific Organic Anion Transporter 1 - metabolism; Male; natural products; Rats; Rats, Sprague-Dawley; Serum Albumin - metabolism; Solute Carrier Organic Anion Transporter Family Member 1B3 - antagonists & inhibitors; Solute Carrier Organic Anion Transporter Family Member 1B3 - metabolism; transporters; Triterpenes - chemistry; Triterpenes - pharmacokinetics; Triterpenes - pharmacology; food interactions; drug interactions; ATV; transporters; CysA; DCF; drug transport; UA; natural products; BSA; LC-MS/MS; OA; DDIs; FBS; DPBS; OATP1B1/3; BA
• ISSN: 0022-3549; 1520-6017
• DOI: 10.1016/j.xphs.2018.02.010

Betulinic acid (BA), a plant-derived pentacyclic triterpenoid, may interact with the members of the organic anion transporting polypeptide 1B subfamily. Here, we investigated the interactions of BA and its analogs with OATP1B1/3 and rat Oatp1b2 in vitro and in vivo. BA inhibited the activity of OATP1B1/3 and rat Oatp1b2 in vitro. Systemic exposure of atorvastatin was substantially altered with the intravenous co-administration of BA (20 mg/kg). Preincubation (incubation with inhibitors, followed by washout) with BA led to a sustained inhibition of OATP1B3, which recovered rapidly in the media containing 10% fetal bovine serum. The addition of albumin to the media decreased intracellular concentrations of BA and expedited the recovery of OATP1B3 activity following preincubation. For asunaprevir and cyclosporin A (previously known to inhibit OATP1B3 upon preincubation), the addition of albumin to the media shortened recovery time with asunaprevir, but not with cyclosporin A. Overall, our results showed that BA inhibits OATP1B transporters in vitro and may incur hepatic transporter-mediated drug interactions in vivo. Our results identify BA as another OATP1B3 inhibitor with preincubation effect and suggest that the preincubation effect and its duration is impacted by altered equilibrium of inhibitors between intracellular and extracellular space (e.g., albumin in the media).