Secretagogues for lysosomal enzyme release as stimulants of arachidonyl phosphatidylinositol turnover in rabbit neutrophils
[ 14C]arachidonic acid incorporation into phosphatidylinositol from rabbit neutrophils pretreated with cytochalasin B was increased within 2 min by the synthetic peptide formyl-methionyl-leucyl-phenylalanine and the Ca 2+ ionophore A23187. A high concentration of the peptide elicited only a small in...
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Published in | Biochemical and biophysical research communications Vol. 90; no. 4; pp. 1364 - 1370 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
29.10.1979
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Subjects | |
Online Access | Get full text |
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Summary: | [
14C]arachidonic acid incorporation into phosphatidylinositol from rabbit neutrophils pretreated with cytochalasin B was increased within 2 min by the synthetic peptide formyl-methionyl-leucyl-phenylalanine and the Ca
2+ ionophore A23187. A high concentration of the peptide elicited only a small increase in phosphatidylinositol turnover when [
14C]palmitic acid was employed as precursor. These data coincide with our earlier studies which identified a Ca
2+-dependent increase in arachidonyl phosphatidylinositol turnover during adrenocorticotropin and A23187-induced activation of steroid production and release in adrenocortical cells. It is concluded that an increase in arachidonyl phosphatidylinositol turnover mediated by a Ca
2+-dependent phospholipase A
2 may be a general mechanism for altering membrane function during secretory activity. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/0006-291X(79)91186-0 |