Estradiol-17β- progesterone and 5α-dihydrotestosterone receptors of uterine myometrium and myoma in the human subject

• Published in: Journal of steroid biochemistry Vol. 10; no. 6; pp. 615 - 622
• Main Authors: Tamaya, Teruhiko, Motoyama, Toshihiko, Ohono, Yousuke, Ide, Norimasa, Tsurusaki, Toshihide, Okada, Hiroji
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.06.1979
• Subjects: Adult; Cytosol - metabolism; Dihydrotestosterone - metabolism; Estradiol - metabolism; Female; Humans; Leiomyoma - metabolism; Mitotic Index; Myometrium - metabolism; Receptors, Androgen - isolation & purification; Receptors, Androgen - metabolism; Receptors, Estrogen - isolation & purification; Receptors, Estrogen - metabolism; Receptors, Progesterone - isolation & purification; Receptors, Progesterone - metabolism; Receptors, Steroid - metabolism; Uterine Neoplasms - metabolism; Uterus - metabolism; Estrogen receptor; estradiol-17β; androgen receptor; dihydrotestosterone; progesterone; dissociation constant; progesterone receptor
• ISSN: 0022-4731
• DOI: 10.1016/0022-4731(79)90513-2

Steroid receptors in human uterine myomas and the corresponding myometria were characterized and investigated to clarify their relation to tumor growth. The 248,800 g supernatant of tissue homogenates was used, in which steroid receptors were characterized and determined by Scatchard plot analysis using dextran coated charcoal at 4°C. In cytosols of both myoma and moymetrium, the estrogen receptors (ER) were sedimented at approximately the 7S, 5S and 4S regions, and the estradiol-17β(E 2)-ER complex had a dissociation constant ( K D ) = 4.5 × 10 −10M. Progesterone receptors (PR) were sedimented at approximately the 7 S and 4–5 S regions. The K D of the progesterone-PR complex was 1.5 × 10 −9M and the K D of the R5020-PR complex was 5.0 × 10 −10M. Androgen receptors (AR) were sedimented at approximately the 6–7 S and 5 S regions and dihydrotestosterone (DHT)-AR complex had a K D = 4.0 × 10 −10M. All of steroid-7 S binding in the cytosol were easily degraded during 5–20% sucrose gradient centrifugation. Ligand specificity studies characterized the specificity of receptors. There was no significant difference in steroid receptor levels (expressed as fmol/mg protein) between myoma and the corresponding myometrium; however if the receptor level was expressed as fmol/μDNA, the estrogen receptor level in the myometrium was higher than that in the myoma ( P < 0.05). There was no significant difference in mitotic index between the two tissues.