Cholesterol side chain cleavage and aromatase activities in the corpus luteum of the pregnant rhesus monkey

• Published in: Steroids Vol. 29; no. 2; pp. 237 - 248
• Main Authors: Sholl, S.A., Wolf, R.C., Colás, A.E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.1977
• Subjects: Animals; Aromatase - metabolism; Cholesterol - metabolism; Cholesterol Side-Chain Cleavage Enzyme - metabolism; Corpus Luteum - metabolism; Female; Haplorhini; In Vitro Techniques; Kinetics; Macaca mulatta; Mitochondria - metabolism; Ovarian Follicle - metabolism; Oxidoreductases - metabolism; Pregnancy; Testosterone - metabolism; androstenedione, 4-andro-stene-3, 17-dione; 17α-hydroxyprogesterone, 17α-hydroxy-4-pregnene-3,20-dione; estradiol-17β, estra-1,3,5-triene-3, 17β-diol; cholesterol, 5-cholesten-3β-o1; pregnenolone, 3β-hydroxy-5-pregnen-20-one; dehydroepiandro-sterone, 3β-hydroxy-5-androsten-17-one; 20α-dihydroprogesterone, 20α-hydroxy-4-pregnen-3-one; progesterone, 4-pregnene-3,20-dione
• ISSN: 0039-128X; 1878-5867
• DOI: 10.1016/0039-128X(77)90042-3

Cholesterol side-chain cleavage (CSCC) and aromatase activities were measured in luteal mitochondria and tissue pieces, respectively, from rhesus monkeys on days 22, 49, 128 and 160 of gestation. CSCC activity did not vary significantly during gestation and thus probably does not respond to chorionic gonadotropin which is elevated on day 22 of pregnancy. It is not known, however, whether CSCC can be stimulated prior to day 22 when the corpus luteum is steroidogenically more active. Both 3H-pregnenolone and 3H-progesterone were synthesized from [1,2- 3H]cholesterol. Aromatase activity declined from high levels on days 22 and 49 to a nadir on day 128 of pregnancy. Utilizing either [1β- 3H]androstenedione or [1β- 3H]testosterone as substrate yielded comparable results throughout gestation.