ZBTB20-AS1 promoted Alzheimer's disease progression through ZBTB20/GSK-3β/Tau pathway

• Published in: Biochemical and biophysical research communications Vol. 640; pp. 88 - 96
• Main Authors: Wang, Yanwen, Cai, Miao, Lou, Yue, Zhang, Siran, Liu, Xiaoli
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.01.2023
• Subjects: Alzheimer Disease - genetics; Alzheimer Disease - metabolism; Alzheimer's disease; Apoptosis; Cell Line, Tumor; Glycogen Synthase Kinase 3 beta - genetics; Glycogen Synthase Kinase 3 beta - metabolism; GSK3β; Humans; LncRNA ZBTB20-AS1; MicroRNAs - genetics; Nerve Tissue Proteins - metabolism; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Tau phosphorylation; tau Proteins - metabolism; Transcription Factors; Tau phosphorylation; GSK3β; Alzheimer's disease; LncRNA ZBTB20-AS1; Apoptosis
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2022.11.107

To elucidate the potential molecular mechanisms of ZBTB20-AS1 on ZBTB20 and GSK-3β/Tau signaling pathway in the pathogenesis of Alzheimer's disease (AD), SH-SY5Y cells were obtained for in vitro experiments and AD models were constructed using β-Amyloid 1–42. CCK8 assay was implemented for determining cell viability. Flow cytometry was used for cell apoptosis detection. Dual-luciferase reporter and RNA-RNA pull down assay was employed for elucidating molecular interactions. Immunohistochemistry, RT-qPCR and western blotting were performed for measuring gene expression. The results showed that expression of LncRNA ZBTB20-AS1 was significantly upregulated, while ZBTB20 was downregulated in SH-SY5Y-AD cells. ZBTB20 was the target gene of LncRNA ZBTB20-AS1. Overexpression of ZBTB20 or knockdown of LncRNA ZBTB20-AS1 inhibited SH-SY5Y-AD cells apoptosis and suppressed GSK3β/Tau pathway, and knockdown of ZBTB20-AS1 increased cell viability and decreased apoptosis. In conclusion, overexpression of ZBTB20-AS1 inhibited ZBTB20 expression and promoted GSK-3β expression and Tau phosphorylation, contributing to the development of AD. •LncRNA ZBTB20-AS1 was upregulated in AD.•miR-132–3p was the target gene of LncRNA ZBTB20-AS1.•ZBTB20 inhibited AD cells apoptosis and suppressed GSK3β/Tau pathway.•Knockdown of LncRNA ZBTB20-AS1 alleviated the progression of AD.