Electrochemical Studies of Cu(phen)edda Interaction with DNA

• Published in: International journal of electrochemical science Vol. 7; no. 5; pp. 4692 - 4701
• Main Authors: Yaw, C.W., Tan, W.T., Tan, W.S., Ng, C.H., Yap, W.B., Rahman, N.H., Zidan, Mohamed
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 2012
• Subjects: [Cu(phen)(edda)]·5H2O; DNA; Interaction; voltammetry; voltammetry; [Cu(phen)(edda)]·5H2O; Interaction; DNA
• ISSN: 1452-3981; 1452-3981
• DOI: 10.1016/S1452-3981(23)19574-5

Copper phenanthroline ethylenediaminediacetic acid, [Cu(phen)(edda)p 5H2O, has been synthesized and characterized using cyclic voltammetry (CV), chronocoulometry (CC), chronoamperometry (CA), and rotating disk electrode (RDE). The copper(II) complex is found to be electroactive as shown by its well defined redox waveforms during cyclic voltammetry at room temperature and at pH 6.8. The interaction between [Cu(phen)(edda)]·5H2O and DNA was also investigated using cyclic voltammetry at room temperature and at pH 6.8. The above electrochemical interaction was evident as their CV results revealing a slight shift in formal potential (at a range of 5 to 23mV dependant on scan rat, and a significant changes in redox peak currents (at a range of 0.05 to 0.4 times dependant on scan rate) to that of Cu(II) complexes in the presence of DNA. Copper (II) complex dissociates more easily as solution acidity increases as is evident by its larger current and decreases in peaks separation. Based on hydrodynamic voltammetric studies, the diffusion coefficients copper complex is found to be of the order of 10-7cm2/s and kinetic rate constant of 10-5cm/s.