Hybrid biofabricated blood vessel for medical devices testing

• Published in: Science and technology of advanced materials Vol. 25; no. 1; p. 2404382
• Main Authors: Portone, Alberto, Ganzerli, Francesco, Petrachi, Tiziana, Resca, Elisa, Bergamini, Valentina, Accorsi, Luca, Ferrari, Alberto, Sbardelatti, Simona, Rovati, Luigi, Mari, Giorgio, Dominici, Massimo, Veronesi, Elena
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 31.12.2024; Taylor & Francis Group
• Subjects: 3D bioprinting; Bio-Inspired and Biomedical Materials; biocompatibility; cytotoxicity assay; fast diffusion-induced gelation; in vitro models; three-layered artificial blood vessel; fast diffusion-induced gelation; biocompatibility; in vitro models; 3D bioprinting; cytotoxicity assay; medical device; three-layered artificial blood vessel
• ISSN: 1468-6996; 1878-5514
• DOI: 10.1080/14686996.2024.2404382

Current and tests applied to assess the safety of medical devices retain several limitations, such as an incomplete ability to faithfully recapitulate human features, and to predict the response of human tissues together with non-trivial ethical aspects. We here challenged a new hybrid biofabrication technique that combines bioprinting and Fast Diffusion-induced Gelation strategy to generate a vessel-like structure with the attempt to spatially organize fibroblasts, smooth-muscle cells, and endothelial cells. The introduction of Fast Diffusion-induced Gelation minimizes the endothelial cell mortality during biofabrication and produce a thin endothelial layer with tunable thickness. Cell viability, Von Willebrand factor, and CD31 expression were evaluated on biofabricated tissues, showing how bioprinting and Fast Diffusion-induced Gelation can replicate human vessels architecture and complexity. We then applied biofabricated tissue to study the cytotoxicity of a carbothane catheter under static condition, and to better recapitulate the effect of blood flow, a novel bioreactor named CuBiBox (Customized Biological Box) was developed and introduced in a dynamic modality. Collectively, we propose a novel bioprinted platform for human biocompatibility testing, predicting the impact of medical devices and their materials on vascular systems, reducing animal experimentation and, ultimately, accelerating time to market.