Long non-coding RNA 319 facilitates nasopharyngeal carcinoma carcinogenesis through regulation of miR-1207-5p/KLF12 axis

• Published in: Gene Vol. 680; pp. 51 - 58
• Main Authors: Song, Peng, Yin, Shu-Cheng
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.01.2019
• Subjects: 3' Untranslated Regions; Animals; Carcinoma - genetics; Carcinoma - metabolism; Cell Line, Tumor; Cell Proliferation; Gene Expression Regulation, Neoplastic; Humans; KLF12; Kruppel-Like Transcription Factors - genetics; Kruppel-Like Transcription Factors - metabolism; LINC00319; Mice; MicroRNAs - genetics; miR-1207-5p; Nasopharyngeal Carcinoma; Nasopharyngeal carcinoma (NPC); Nasopharyngeal Neoplasms - genetics; Nasopharyngeal Neoplasms - metabolism; Neoplasm Transplantation; Prognosis; RNA, Long Noncoding - genetics; Tumorigenesis; lncRNAs; NPC; CC; ceRNA; KLF12; NSCLC; IHC; miR-1207-5p; GBC; EMT; Nasopharyngeal carcinoma (NPC); RCC; ANOVA; LINC00319; Tumorigenesis; GC; GAPDH
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2018.09.032

Long non-coding RNAs (lncRNAs) have been wildly verified to modulate multiple tumorigeneses, especially nasopharyngeal carcinoma (NPC). In present study, we aim to investigate the role of LINC00319 in the NPC carcinogenesis. It was indicated that LINC00319 was markedly increased in NPC tissues and cells in comparison to their corresponding controls. Moreover, the aberrant overexpression of LINC00319 indicated the poor prognosis of NPC patients. Silence of LINC00319 was able to suppress NPC cell growth in vitro while overexpression of LINC00319 inversed this process. Moreover, in vivo tumor xenografts were established using CNE-1/SUNE-1 cells to investigate the function of LINC00319 in NSCLC tumorigenesis. Rescue assay was performed to further confirm that LINC00319 contributed to NPC progression by regulating miR-1207-5p/KLF12 signal pathway. Taken together, our study discovered the oncogenic role of LINC00319 in clinical specimens and cellular experiments, showing the potential LINC00319/miR-1207-5p/KLF12 pathway. This results and findings provide a novel insight for NPC tumorigenesis. •LINC00319 is up-regulated in human primary NPC tissues.•Expression of KLF12 is up-regulated in primary human NPC and negatively expressed related to miR-1207-5p.•miR-1207-5p inhibits the tumorigenic potential of NPC cells by down-regulating oncogenic KLF12 gene.•LINC00319's oncogenic functions are partially through reverse regulation of miR-1207-5p, and then activation of KLF12.