Qualitative analysis on the transdermal absorption from the dichloromethane extract of the Sambucus adnata wall. based on UPLC-Q-Exactive Orbitrap/MS

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 234; p. 115509
• Main Authors: Qin, Shu-ran, Wang, Wei, Li, Dashan, Pu, Yue-fei, Cui, Tao, Wang, Wen-jing
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 20.09.2023
• Subjects: Chemical constituents; Dichloromethane extract; Sambucus adnata Wall; Transdermal penetration; UPLC-Q-Exactive Orbitrap/MS; UPLC-Q-Exactive Orbitrap/MS; Sambucus adnata Wall; Transdermal penetration; Dichloromethane extract; Chemical constituents
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2023.115509

Sambucus adnata Wall.(SAW) has been used to treat osteoarthritis by the Yi nationality in China. The present study established an overall identification strategy based on ultra-high performance liquid chromatography-tandem Q-Exactive Orbitrap mass spectrometry (UPLC-Q-Exactive Orbitrap/MS) method to characterize the multiple chemical constituents of SAW before and after percutaneous penetration. Nineteen compounds, including triterpenoids, fatty acids, lignans, flavonoid, and amide, were tentatively identified in the dichloromethane extract of SAW, while fourteen ingredients penetrated the skin. Among them, eleven components were reported for the first time in SAW. •Qualitative analysis on the transdermal absorption of SAW-DCE by LC-MS.•Nineteen constituents were identified in SAW-DCE.•Fourteen ingredients were identified after penetrating the skin.•Eleven compounds were reported for the first time in SAW.