Improvements in the Extractive and Carbohydrate Analysis of Sugarcane Bagasse

• Published in: Waste and biomass valorization Vol. 12; no. 7; pp. 3727 - 3740
• Main Authors: Barbosa, Paula S., Barbosa, Marcio H. P., de Faria, Bruno de F. H., Teófilo, Reinaldo F.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.07.2021; Springer Nature B.V
• Subjects: Acetonitrile; Aqueous solutions; Arabinose; Bagasse; Biomass; Carbohydrate analysis; Carbohydrates; Design of experiments; Design optimization; Engineering; Environment; Environmental Engineering/Biotechnology; Ethanol; Galactans; Galactose; Industrial Pollution Prevention; Ions; Liquid chromatography; Mannose; Original Paper; Polysaccharides; Renewable and Green Energy; Retention time; Sugarcane; Waste Management/Waste Technology; Carbohydrates; Extractives; Sugarcane; Lignocellulosic biomass; Holocellulose
• ISSN: 1877-2641; 1877-265X
• DOI: 10.1007/s12649-020-01268-y

Generic procedures for the determination of extractives and carbohydrates (glucose, xylose, galactose, arabinose, mannose) were improved specifically for sugarcane bagasse. The extraction time was optimized using a specific design of experiments. The optimal condition for sugarcane biomass with up to 20% extractives was 11.5 h of extraction in water, followed by 9 h in ethanol. For the carbohydrate analysis, a separation method using liquid chromatography was improved and optimized to reduce the retention time of furanic compounds. The reduction obtained was approximately 30%. The optimal condition was the isocratic elution of the mobile phase with an aqueous solution containing 15% acetonitrile. The percentages of glycans, xylans, and arabinans in the extractive-free biomass was 33%, 22%, and 6.5%, respectively. The galactans and mannans were not detected. Acetyl groups and uronic acids were not determined. Graphic Abstract