Transplantation of insulin-secreting cells differentiated from human adipose tissue-derived stem cells into type 2 diabetes mice

• Published in: Biochemical and biophysical research communications Vol. 443; no. 2; pp. 775 - 781
• Main Authors: Nam, Ji Sun, Kang, Hyun Mi, Kim, Jiyoung, Park, Seah, Kim, Haekwon, Ahn, Chul Woo, Park, Jin Oh, Kim, Kyung Rae
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.01.2014
• Subjects: Adipocytes - pathology; Adipose tissue-derived stem cells; Animals; Blood Glucose - metabolism; Cell Differentiation; Cells, Cultured; Diabetes Mellitus, Type 2 - metabolism; Diabetes Mellitus, Type 2 - surgery; Female; Humans; Insulin - blood; Insulin-secreting cells; Insulin-Secreting Cells - pathology; Insulin-Secreting Cells - transplantation; Interleukin-6; Metabolic parameters; Mice; Mice, Inbred C57BL; Stem Cells - pathology; Treatment Outcome; Type 2 diabetes; Type 2 diabetes; Metabolic parameters; UDC; hEA-ISCs; Insulin-secreting cells; Adipose tissue-derived stem cells; DC; hEAs; Interleukin-6; insulin secreting cells differentiated from human eyelid adipose-derived stem cells; undifferentiated cell; differentiated cell; human eyelid adipose-derived stem cells
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2013.10.059

•hEA-ISCs transplantation increased human insulin, c-peptide levels in T2DM mouse.•hEA-ISCs improved blood glucose level and survival in T2DM mouse.•Metabolic parameters and IL-6 were ameliorated by hEA-ISC. Currently, there are limited ways to preserve or recover insulin secretory capacity in human pancreas. We evaluated the efficacy of cell therapy using insulin-secreting cells differentiated from human eyelid adipose tissue-derived stem cells (hEAs) into type 2 diabetes mice. After differentiating hEAs into insulin-secreting cells (hEA-ISCs) in vitro, cells were transplanted into a type 2 diabetes mouse model. Serum levels of glucose, insulin and c-peptide were measured, and changes of metabolism and inflammation were assessed in mice that received undifferentiated hEAs (UDC group), differentiated hEA-ISCs (DC group), or sham operation (sham group). Human gene expression and immunohistochemical analysis were done. DC group mice showed improved glucose level, and survival up to 60days compared to those of UDC and sham group. Significantly increased levels of human insulin and c-peptide were detected in sera of DC mice. RT-PCR and immunohistochemical analysis showed human gene expression and the presence of human cells in kidneys of DC mice. When compared to sham mice, DC mice exhibited lower levels of IL-6, triglyceride and free fatty acids as the control mice. Transplantation of hEA-ISCs lowered blood glucose level in type 2 diabetes mice by increasing circulating insulin level, and ameliorating metabolic parameters including IL-6.