Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells

• Published in: Neural regeneration research Vol. 7; no. 18; pp. 1370 - 1377
• Main Authors: Zheng, Jinghui, Liang, Jian, Deng, Xin, Chen, Xiaofeng, Wu, Fasheng, Zhao, Xiaofang, Luo, Yuan, Fu, Lei, Jiang, Zuling
• Format: Journal Article
• Language: English
• Published: India Medknow Publications & Media Pvt. Ltd 25.06.2012; Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Re; Medknow Publications & Media Pvt Ltd
• Subjects: Bone marrow; Chromatography; Kinases; Laboratory animals; Medical research; Morphology; p38丝裂原活化蛋白激酶; Pharmacy; Protein expression; Proteins; R&D; Research & development; Research and Report: Traditional Chinese Medicine and Neural Regeneration; Signal transduction; Stem cells; Traditional Chinese medicine; 信号通路; 神经元特异性烯醇化酶; 细胞分化; 补阳还五汤; 间质; 骨髓基质; China; neural regeneration; neuron specific enolase; nestin; mitogen-activated protein kinase signaling pathway; bone marrow mesenchymal stem cells; extracellular signal-regulated protein kinase; Buyang Huanwu decoction; cell signal transduction pathway
• ISSN: 1673-5374; 1876-7958
• DOI: 10.3969/j.issn.1673-5374.2012.18.002

Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell differentiation into neurons. Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process. The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation. Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively, mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction. Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.