Membrane-acting bacteriocin purified from a soil isolate Pediococcus pentosaceus LB44 shows broad host-range

• Published in: Biochemical and biophysical research communications Vol. 498; no. 4; pp. 810 - 816
• Main Authors: Kaur, Ramanjeet, Tiwari, Santosh Kumar
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.04.2018
• Subjects: Amino Acid Sequence; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antimicrobial activity; Bacteria - drug effects; Bacterial Infections - drug therapy; Bacteriocin LB44; Bacteriocins - chemistry; Bacteriocins - isolation & purification; Bacteriocins - pharmacology; Food Microbiology; FTIR spectroscopy; Halobacteriaceae - drug effects; Humans; Mode of action; Pediococcus pentosaceus - chemistry; Pediococcus pentosaceus LB44; Soil Microbiology; Bacteriocin LB44; Pediococcus pentosaceus LB44; Antimicrobial activity; Mode of action; FTIR spectroscopy
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2018.03.062

Bacteriocin LB44 was purified from cell-free supernatant (CFS) of Pediococcus pentosaceus LB44 using activity-guided chromatography techniques. It was stable up to 121 °C, pH 2.0–6.0, sensitive to proteinase K, papain and trypsin, and retained complete activity in the presence of organic solvents tested. The molecular weight of bacteriocin was ∼6 kDa and initial ten amino acid residues (GECGMCXECG) suggested a new compound. The loss in viable cell count and K+ ion efflux of target cells of Micrococcus luteus suggested bactericidal activity. The cell membrane of bacteriocin-treated cells was found to be ruptured which was further confirmed by Fourier Transform Infrared (FTIR) analysis suggesting interaction of bacteriocin with phospholipids in cell membrane. It showed broad host-range and inhibited the growth of Lactobacillus delbrueckii NRRL B-4525, L. plantarum NRRL B-4496, L. acidophilus NRRL B-4495, Enterococcus hirae LD3, Weissella confusa LM85, Staphylococcus aureus, Salmonella typhi ATCC 13311, Serratia marcescens ATCC 27137, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 29905, Haloferax larsenii HA1, HA3, HA8, HA9 and HA10. These properties suggested a new bacteriocin from soil isolate P. pentosaceus LB44 which may offers possible applications in food-safety and therapeutics. •Bacteriocin LB44 was purified from soil isolate Pediococcus pentosaceus LB44 using activity-guided chromatography.•It was found to be heat stable, active in acidic conditions and sensitive to proteinase K, papain and trypsin.•Molecular weight was estimated to be ∼6 kDa.•The Fourier Transform Infrared (FTIR) analysis, K+ ion efflux and scanning electron microscopy of target cells suggested membrane-acting bactericidal activity.•Bacteriocin LB44 showed broad host range against Gram-positive, Gram-negative and haloarchaea strains tested.