Studies on anti-angiogenesis of ginsenoside structure modification HRG in vitro

• Published in: Biochemical and biophysical research communications Vol. 492; no. 3; pp. 391 - 396
• Main Authors: Li, Ji-ping, Zhao, Feng-li, Yuan, Ye, Sun, Ting-ting, Zhu, Lei, Zhang, Wen-you, Liu, Mo-xiang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.10.2017
• Subjects: Angiogenesis; Cell proliferation; Cell Proliferation - drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Ginsenoside structure modification; Ginsenosides - chemistry; Human Umbilical Vein Endothelial Cells - drug effects; Humans; HUVECs; Migration; Molecular Conformation; Neovascularization, Pathologic - drug therapy; Saponins - chemical synthesis; Saponins - chemistry; Saponins - pharmacology; Structure-Activity Relationship; VEGF; Ginsenoside structure modification; Cell proliferation; Angiogenesis; Migration; VEGF; HUVECs
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2017.08.090

This study investigates the anti-angiogenic effect of 3β, 12β, 20(S)-trihydroxy dammarane-3-O-β-d-glucopyranosyl(1–2)-β-d-glucopyranoside(HRG), a new chemical compound obtained by structure modification on Ginseng saponins Rg3, associated with the regulation of matrix metalloproteinases(MMPs) and its upstream signal-regulated molecule of vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(b-FGF) in vitro, which plays an critical role in angiogenesis during the process of carcinoma. In our study, to investigate the anti-angiogenesis effect of HRG in HUVECs, we utilized cell proliferation assay, tube formation assay, wound-healing assay, Semi-quantitative reverse transcription PCR, and Western blot assay. Our results demonstrated that HRG plays a major role in the regulation of proliferation, migration and tube formation of HUVECs by suppressing the expression of VEGF and b-FGF in both transcriptional and post-transcriptional levels. In addition, the expression of MMP-2 and MMP-9, which were related to the ECM degradation, were down-regulated after administration of HRG as well. Overall, our results revealed that HRG strongly inhibited the process of angiogenesis and shows better effectiveness than Rg3.