Methylation analysis of the carbohydrate portion of fibronectin isolated from human plasma

Fibronectin was isolated from pooled human plasma by affinity chromatography on Sepharose-coupled gelatin and subjected to methylation analysis. The results indicate that the carbohydrate chains in fibronectin are very similar to those found in other serum glycoproteins, i.e. a common inner core str...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 84; no. 1; pp. 269 - 274
Main Author Wrann, Michael
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 14.09.1978
Subjects
Carbohydrates - analysis
Glycoproteins - isolation & purification
Glycosides - analysis
Humans
Mass Spectrometry
Membrane Proteins - isolation & purification
Methylation
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Summary:Fibronectin was isolated from pooled human plasma by affinity chromatography on Sepharose-coupled gelatin and subjected to methylation analysis. The results indicate that the carbohydrate chains in fibronectin are very similar to those found in other serum glycoproteins, i.e. a common inner core structure consisting of two N-acetylglucosamine and three mannose residues attached to the polypeptide backbone via a N-acetylglucosamine-asparagine linkage. To this inner core are attached two side chains made up of N-acetylglucosamine, galactose and terminated by sialic acid.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(78)90292-9