CMV70-3P miRNA contributes to the CMV mediated glioma stemness and represents a target for glioma experimental therapy

• Published in: Oncotarget Vol. 8; no. 16; pp. 25989 - 25999
• Main Authors: Ulasov, Ilya V, Kaverina, Natalya V, Ghosh, Dhimankrishna, Baryshnikova, Marya A, Kadagidze, Zaira G, Karseladze, Apollon I, Baryshnikov, Anatoly Y, Cobbs, Charles S
• Format: Journal Article
• Language: English
• Published: United States Impact Journals LLC 18.04.2017
• Subjects: AC133 Antigen - metabolism; Cell Line, Tumor; Cell Movement - genetics; Cytomegalovirus - physiology; Cytomegalovirus Infections - complications; Cytomegalovirus Infections - virology; Gene Expression; Glioma - drug therapy; Glioma - etiology; Glioma - metabolism; Glioma - pathology; Humans; MicroRNAs; Neoplastic Stem Cells - metabolism; Phenotype; Promoter Regions, Genetic; Research Paper; RNA, Viral; SOXB1 Transcription Factors - genetics; brain tumor; miRNA; cytomegalovirus; glioma stem cells
• ISSN: 1949-2553; 1949-2553
• DOI: 10.18632/oncotarget.11175

Glioblastoma multiforme (GBM) is a rapidly progressive brain tumor with a median survival of 15-19 months. Therapeutic resistance and recurrence of the disease is attributed to cancer stem cells (CSC). Here, we report that CMV70-3P miRNA encoded by CMV increases GBM CSC stemness. Inhibition of CMV70-3P expression using oligo inhibitors significantly attenuated the ability of primary glioma cells to proliferate and form neurospheres. At the molecular level, we show that CM70-3P increases expression of cellular SOX2. Collectively, these findings indicate that CMV70-3P is a potential regulator of CMV- mediated glioma progression and cancer stemness.