Studies on synthesis and structure of virus-coded low molecular weight RNA in adenovirus type 2-infected KB cells

Three new virus-coded low molecular weight RNA species which migrate slightly faster on two-dimensional polyacrylamide gels than virus-associated (VA) RNA were isolated from KB cells late after productive infection with adenovirus type 2. All three species are synthesized in quantities much smaller...

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Bibliographic Details
Published inVirology (New York, N.Y.) Vol. 74; no. 2; pp. 386 - 393
Main Authors Varricchio, Frederick, Sehulster, Lynne M., Raška, Karel
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.10.1976
Subjects
Adenoviridae - metabolism
Cell Line
Kinetics
Nucleic Acid Conformation
Oligonucleotides - analysis
Ribonucleases
RNA, Viral - analysis
RNA, Viral - biosynthesis
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Summary:Three new virus-coded low molecular weight RNA species which migrate slightly faster on two-dimensional polyacrylamide gels than virus-associated (VA) RNA were isolated from KB cells late after productive infection with adenovirus type 2. All three species are synthesized in quantities much smaller than VA RNA. The relative rates of labeling of the three species differ at different times after infection. The base composition of the three novel RNA molecules differs among themselves and is different from that of VA RNA. The structure of the minor RNA species with migration most different from that of VA RNA was studied in detail. Its T 1 ribonuclease oligonucleotide map resembles the map of VA RNA but is not identical. It has a different 3′ end from VA RNA and is missing several T 1 oligonucleotides present in our VA RNA. It contains some T 1 oligonucleotides not present in VA RNA.
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ISSN:0042-6822
1096-0341
DOI:10.1016/0042-6822(76)90344-5