Schisandrin B protects PC12 cells by decreasing the expression of amyloid precursor protein and vacuolar protein sorting 35

• Published in: Neural regeneration research Vol. 7; no. 9; pp. 652 - 658
• Main Authors: Yan, Mingmin, Mao, Shanping, Dong, Huimin, Liu, Baohui, Zhang, Qian, Pan, Gaofeng, Fu, Zhiping
• Format: Journal Article
• Language: English
• Published: India Medknow Publications & Media Pvt. Ltd 25.03.2012; Department of Neurology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China%Department of Neurosurgery, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China; Medknow Publications & Media Pvt Ltd
• Subjects: Apoptosis; Drug dosages; Kinases; PC12细胞; Protein expression; Proteins; Research and Report: Traditional Chinese Medicine and Neuroregeneration; 五味子乙素; 保护; 排序; 液泡; 淀粉样前体蛋白; 淀粉样蛋白; 逆转录PCR; China; vacuolar protein sorting 35; Schisandrin B; amyloid β-protein 25–35; amyloid precursor protein; PC12 cells; neural protection; amyloid β-protein 25-35
• ISSN: 1673-5374; 1876-7958
• DOI: 10.3969/j.issn.1673-5374.2012.09.002

PC12 cell injury was induced using 20 μM amyloid β-protein 25-35 to establish a model of Alzheimer＇s disease. The cells were then treated with 5, 10, and 25 μM Schisandrin B. Methylthiazolyldiphenyl-tetrazolium bromide assays and Hoechst 33342 staining results showed that with increasing Schisandrin B concentration, the survival rate of PC12 cells injured by amyloid β-protein 25-35 gradually increased and the rate of apoptosis gradually decreased. Reverse transcription-PCR, immunocytochemical staining and western blot results showed that with increasing Schisandrin B concentration, the mRNA and protein expression of vacuolar protein sorting 35 and amyloid precursor protein were gradually decreased. Vacuolar protein sorting 35 and amyloid precursor protein showed a consistent trend for change. These findings suggest that 5, 10, and 25 μM Schisandrin B antagonizes the cellular injury induced by amyloid β-protein 25-35 in a dose-dependent manner. This may be caused by decreasing the expression of vacuolar protein sorting 35 and amyloid precursor protein.