Comparative mutagenesis of plant flavonoids in microbial systems

• Published in: Mutation Research/Genetic Toxicology Vol. 58; no. 2; pp. 231 - 239
• Main Authors: Hardigree, A.A., Epler, J.L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.1978
• Subjects: Animals; Flavonoids - pharmacology; Genetic Techniques; Mutagens; Nicotiana; Plants; Plants, Toxic; Rats; Rutin - pharmacology; Salmonella typhimurium - genetics
• ISSN: 0165-1218; 0027-5107
• DOI: 10.1016/0165-1218(78)90014-9

The plant flavonoids quercetin (3,5,7,3′,4′-pentahydroxyflavone), morin (3,5,7,2′,4′-pentahydroxyflavone), kaempferol (3,5,7,4′-tetrahydroxyflavone), chrysin (5,7-dihydroxyflavone), fisetin (3,7,3′,4′-tetrahydroxyflavone), myricetin (3,5,7,3′,4′,5′-hexahydroxyflavone), myricitrin (myricetin-3-rhamnoside), hesperetin (3′,5,7-trihydroxy-4′-methoxyflavanone), quercitrin (quercetin-3- l-rhamnoside), rutin (quercetin-3-rhamnosylglucoside or quercetin-3-rutinoside), and hesperidin (hesperetin-7-rutinoside) have been assayed for mutagenicity in the Salmonella/microsomal activation system. Quercetin, morin, kaempferol, fisetin, myricetin, quercitrin and rutin were mutagenic in the histidine reversion system with the frameshift strain TA98. The flavonols quercetin and myricetin are mutagenic without metabolic activation, although more effective when a rat liver microsomal preparation (S-9) is included; all others require metabolic activation. Flavonoids are common constituents of higher plants, with extensive medical uses. In addition to pure compounds, we have examined crude extracts of tobacco (snuff) and extracts from commonly available nutritional supplements containing rutin. Mutagenic activity can be detected and is correlated with the flavonoid content.