Shuanghuanglian injection downregulates nuclear factor-kappa B expression in mice with viral encephalitis

• Published in: Neural regeneration research Vol. 7; no. 33; pp. 2592 - 2599
• Main Authors: Gu, Naibing, Tian, Ye, Di, Zhengli, Han, Caiping, Lei, Hui, Zhang, Gejuan
• Format: Journal Article
• Language: English
• Published: India Medknow Publications & Media Pvt. Ltd 25.11.2012; Department of Neurology,Xi'an Center Hospital,Xi'an 710003,Shaanxi Province,China; Medknow Publications & Media Pvt Ltd
• Subjects: Brain; Encephalitis; Gene expression; Inflammation; Inflammatory diseases; Laboratory animals; Medical research; mRNA水平; mRNA表达; Pathogenesis; Protein expression; Proteins; Research and Report: Traditional Chinese Medicine and Neural Regeneration; 双黄连注射液; 小鼠模型; 核因子-κB; 病毒性脑炎; 蛋白表达; 逆转录PCR; China; neural regeneration; viral encephalitis; short hairpin RNA; nervous system disease; nuclear factor-kappa B; traditional Chinese medicine; intelectin; mice; Shuanghuanglian injection; lentivirus
• ISSN: 1673-5374; 1876-7958
• DOI: 10.3969/j.issn.1673-5374.2012.33.004

A mouse model of viral encephalitis was induced by intracranial injection of a Coxsackie virus B3 suspension. Quantitative real-time reverse transcription-PCR and western blot assay were applied to detect mRNA and protein expression of intelectin-2 and nuclear factor-kappa B in the viral encephalitis and control groups. Nuclear factor-kappa B and intelectin-2 mRNA and protein expression were significantly increased in mice with viral encephalitis. After intraperitoneal injection of Shuanghuanglian at a dose of 1.5 mg/kg for 5 successive days, intelectin-2 and nuclear factor-kappa B protein and mRNA expression were significantly decreased. To elucidate the relationship between intelectin-2 and nuclear factor-kappa B, mice with viral encephalitis were administered an intracerebral injection of 107 pfu recombinant lentivirus expressing intelectin shRNA. Both protein and mRNA levels of intelectin and nuclear factor-kappa B in brain tissue of mice were significantly decreased. Experimental findings suggest that Shuanghuanglian injection may downregulate nuclear factor-kappa B production via suppression of intelectin production, thus inhibiting inflammation associated with viral encephalitis.