Gastrodin blocks neural stem cell differentiation into glial cells mediated by kainic acid

• Published in: Neural regeneration research Vol. 7; no. 12; pp. 891 - 895
• Main Authors: Sun, Guifang, Yuan, Zhihao, Zhang, Boai, Jia, Yanjie, Ji, Yangfei, Ma, Xingrong, Liu, Yu, Liu, Yanru, Wen, Quanqing, Zhao, Yanling
• Format: Journal Article
• Language: English
• Published: India Medknow Publications & Media Pvt. Ltd 25.04.2012; Department of Neurology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China%Department of Urology, the Third People's Hospital of Zhengzhou, Zhengzhou 450051, Henan Province, China%Department of Neurology, the Central Hospital of Nanyang, Nanyang 473009, Henan Province, China; Medknow Publications & Media Pvt Ltd
• Subjects: Amino acids; Antigens; Brain research; Cell culture; Cloning; Enzymes; Fibroblasts; Growth factors; Hazardous materials; Laboratory animals; Nervous system; Neurons; Research and Report: Traditional Chinese Medicine and Neuroregeneration; Stem cells; Tumor necrosis factor-TNF; 介导; 兴奋性氨基酸; 天麻素; 神经干细胞; 神经胶质细胞; 红藻氨酸; 细胞分化; 细胞治疗; United States > US; China; gastrodin; differentiation; kainic acid; neural stem cells
• ISSN: 1673-5374; 1876-7958
• DOI: 10.3969/j.issn.1673-5374.2012.12.002

Kainic acid can simulate excitatory amino acids in vitro. Neural stem cells, isolated from newborn Wistar rats, were cultured in vitro and exposed to 100 4 000 #M kainic acid for 7 days to induce neuronal cell differentiation, causing the number of astrocytes to be significantly increased. Treatment with a combination of 0.5 mg/L gastrodin and kainic acid also caused the number of differentiated neurons to be significantly increased compared with treatment with kainic acid alone Experimental findings suggest that gastrodin reduces the excitability of kainic acid and induces neural stem cell differentiation into neurons.