Identification and characterization of a pyridoxal 5′-phosphate phosphatase in the silkworm (Bombyx mori)

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol. 205; pp. 39 - 45
• Main Authors: Huang, ShuoHao, Han, CaiYun, Ma, ZhenQiao, Zhou, Jie, Zhang, JianYun, Huang, LongQuan
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.03.2017
• Subjects: Animals; Biocatalysis; Bombyx - cytology; Bombyx - enzymology; Bombyx - genetics; Bombyx - metabolism; Bombyx mori; Characterization; Chromosomes - metabolism; Cloning, Molecular; Down-Regulation - genetics; Genomics; Humans; Identification; Larva - genetics; Phosphoric Monoester Hydrolases - chemistry; Phosphoric Monoester Hydrolases - deficiency; Phosphoric Monoester Hydrolases - genetics; Phosphoric Monoester Hydrolases - metabolism; Protein Transport; Pyridoxal 5′-phosphate phosphatase; Pyridoxal Phosphate - metabolism; RNA, Small Interfering - genetics; Characterization; Pyridoxal 5′-phosphate phosphatase; Bombyx mori; Identification
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpb.2017.01.003

Vitamin B6 comprises six interconvertible pyridine compounds, among which pyridoxal 5′-phosphate (PLP) is a coenzyme for over 140 enzymes. PLP is also a very reactive aldehyde. The most well established mechanism for maintaining low levels of free PLP is its dephosphorylation by phosphatases. A human PLP-specific phosphatase has been identified and characterized. However, very little is known about the phosphatase in other living organisms. In this study, a cDNA clone of putative PLP phosphatase was identified from B. mori and characterized. The cDNA encodes a polypeptide of 343 amino acid residues, and the recombinant enzyme purified from E. coli exhibited properties similar to that of human PLP phosphatase. B. mori has a single copy of the PLPP gene, which is located on 11th chromosome, spans a 5.7kb region and contains five exons and four introns. PLP phosphatase transcript was detected in every larva tissue except hemolymph, and was most highly represented in Malpighian tube. We further down-regulated the gene expression of the PLP phosphatase in 5th instar larvae with the RNA interference. However, no significant changes in the gene expression of PLP biosynthetic enzymes and composition of B6 vitamers were detected as compared with the control.