Cyclic AMP-binding proteins in normal and virus-transformed fibroblasts

We examined the notion that altered growth regulation in virally transformed fibroblasts is due to a biochemical lesion affecting cytosol receptor for cyclic AMP. Normal cultured fibroblasts used after two passages and transformed cells from mouse and hamster possess equivalent amounts of total cycl...

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Published inArchives of biochemistry and biophysics Vol. 180; no. 2; pp. 258 - 263
Main Authors Wigglesworth, N.M., Mastro, A., Bourne, H.R., Rozengurt, E.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 30.04.1977
Subjects
Animals
Cell Line
Cell Transformation, Neoplastic
Cricetinae
Cyclic AMP - metabolism
Cytosol - metabolism
Fibroblasts - analysis
Fibroblasts - enzymology
Mice
Protein Kinases - analysis
Receptors, Cyclic AMP - isolation & purification
Simian virus 40
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Summary:We examined the notion that altered growth regulation in virally transformed fibroblasts is due to a biochemical lesion affecting cytosol receptor for cyclic AMP. Normal cultured fibroblasts used after two passages and transformed cells from mouse and hamster possess equivalent amounts of total cyclic AMP receptors, with similar apparent affinities for cyclic AMP and similar distribution among isoenzymic forms of cyclic AMP-dependent protein kinase. Furthermore, untransformed 3T3 cells display only a single peak of cyclic AMP-binding activity, as resolved by ion-exchange chromatography. These findings do not support the view that defective binding of cyclic AMP is essential for loss of growth control in vitro.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0003-9861
1096-0384
DOI:10.1016/0003-9861(77)90036-4