Host-cell reactivation of alkylated T7 bacteriophage

• Published in: Biochimica et biophysica acta Vol. 564; no. 3; p. 495
• Main Authors: Lane, D, Mamet-Bratley, M D, Karska-Wysocki, B
• Format: Journal Article
• Language: English
• Published: Netherlands 25.10.1979
• Subjects: Alkylation; DNA Polymerase I - metabolism; DNA Repair; Endonucleases - metabolism; Escherichia coli - drug effects; Escherichia coli - metabolism; Genotype; Kinetics; Methyl Methanesulfonate - pharmacology; Species Specificity; T-Phages - drug effects; T-Phages - metabolism

Purified T7 phage, treated with methyl methanesulfonate, was assayed on Escherichia coli K-12 host cells deficient in base excision repair. Phage survival, measured immediately after alkylation or following incubation to induce depurination, was lowest on a mutant defective in the polymerase activit...