Host-cell reactivation of alkylated T7 bacteriophage

Purified T7 phage, treated with methyl methanesulfonate, was assayed on Escherichia coli K-12 host cells deficient in base excision repair. Phage survival, measured immediately after alkylation or following incubation to induce depurination, was lowest on a mutant defective in the polymerase activit...

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Bibliographic Details
Published inBiochimica et biophysica acta Vol. 564; no. 3; p. 495
Main Authors Lane, D, Mamet-Bratley, M D, Karska-Wysocki, B
Format Journal Article
LanguageEnglish
Published Netherlands 25.10.1979
Subjects
Alkylation
DNA Polymerase I - metabolism
DNA Repair
Endonucleases - metabolism
Escherichia coli - drug effects
Escherichia coli - metabolism
Genotype
Kinetics
Methyl Methanesulfonate - pharmacology
Species Specificity
T-Phages - drug effects
T-Phages - metabolism
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Summary:Purified T7 phage, treated with methyl methanesulfonate, was assayed on Escherichia coli K-12 host cells deficient in base excision repair. Phage survival, measured immediately after alkylation or following incubation to induce depurination, was lowest on a mutant defective in the polymerase activity of DNA polymerase I (p3478). Strains defective in endonuclease for apurinic sites (AB3027, BW2001) gave a significantly higher level of phage survival, as did the strain defective in the 5'--3' exonuclease activity of DNA polymerase I (RS5065). Highest survival of alkylated T7 phage was observed on the two wild-type strains (AB1157, W3110). These results show that alkylated T7 phage is subject to repair via the base excision repair pathway.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0006-3002
DOI:10.1016/0005-2787(79)90039-X