Biogenic silver nanoparticles synthesized from Rhododendron ponticum and their antibacterial, antibiofilm and cytotoxic activities

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 179; p. 112993
• Main Authors: Nesrin, Korkmaz, Yusuf, Ceylan, Ahmet, Karadağ, Ali, Savaş Bülbül, Muhammad, Nauman Aftab, Suna, Saygılı, Fatih, Şen
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.02.2020
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Anticancer activity; Antineoplastic Agents - pharmacology; Biofilm; Biofilms - drug effects; Cell Line, Tumor; Female; Green synthesis; Humans; Metal Nanoparticles - chemistry; Mice; Microbial Sensitivity Tests; Microscopy, Electron, Transmission; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Rhododendron - chemistry; Rhododendron ponticum; Silver - chemistry; Silver - pharmacology; Silver nanoparticles; Spectrophotometry; X-Ray Diffraction; Biofilm; Antibacterial activity; Green synthesis; Rhododendron ponticum; Silver nanoparticles; Anticancer activity
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2019.112993

•Rhododendron ponticum extracts were used for the biosynthesis of silver nanoparticles•The effects of inhibition of these compounds have been determined in dosage against various bacterial and cancer cell lines.•The evaluation of their antibacterial activity and antibiofilm inhibition against various human pathogenic bacteria. Nanotechnology is being used effectively in many areas contemporarily. Silver nanoparticles (AgNPs) are one of the most crucial and remarkable nanomaterials involved in medical applications. These nanoparticles (NPs) have an important place in nanomedicine, nanotechnology, and in particularly, nanoscience. AgNPs are one of the most widely used materials in antibacterial and antiseptic practices. The synthesis of biogenic AgNPs has been applied as an alternative to physical and chemical synthesis. For this purpose, water extracts of Rhododendron ponticum were used for biosynthesis of AgNPs. Also, AgNPs were characterized by UV–vis spectrophotometer, scanning transmission electron microscope (STEM) and X-ray diffractometer (XRD). The antimicrobial activity of AgNPs synthesized with Rhododendron ponticum was analyzed by the Minimum Inhibition Concentration (MIC) test. Also, the biofilm inhibition test was made, and AgNPs showed a strong effect for biofilm inhibition. In addition, the prepared nanoparticles were tried for cytotoxicity activity with the help of MTT assay in MCF-7 and 4T1 cancer cell lines.