MicroRNA-134-3p is a novel potential inhibitor of human ovarian cancer stem cells by targeting RAB27A

• Published in: Gene Vol. 605; pp. 99 - 107
• Main Authors: Chang, Cui, Liu, Te, Huang, Yongyi, Qin, Wenxing, Yang, Hongtu, Chen, Juan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 20.03.2017
• Subjects: Adenocarcinoma, Clear Cell - genetics; Adenocarcinoma, Clear Cell - metabolism; Adenocarcinoma, Clear Cell - pathology; Adult; Aged; Animals; Base Sequence; Binding Sites; Cell Proliferation; DLK1-DIO3 microRNA cluster; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Genes, Reporter; Humans; Luciferases - genetics; Luciferases - metabolism; Mice; Mice, Nude; MicroRNA-134-3p (miR-134-3p); MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; Neoplasm Invasiveness; Neoplasm Staging; Neoplasm Transplantation; Neoplastic Stem Cells - metabolism; Neoplastic Stem Cells - pathology; Ovarian cancer stem cells (OCSCs); Ovarian Neoplasms - genetics; Ovarian Neoplasms - metabolism; Ovarian Neoplasms - pathology; Primary Cell Culture; rab GTP-Binding Proteins - genetics; rab GTP-Binding Proteins - metabolism; rab27 GTP-Binding Proteins; RAB27A; Signal Transduction; Meg3; miRNAs; Rtl1/Mart1; DMSO; Dio3; RAB27A; mESCs; Ovarian cancer stem cells (OCSCs); MTT; ncRNAs; iPS; Dlk1; snoRNA; MicroRNA-134-3p (miR-134-3p); Gtl2; Mirg; OCSCs; Rtl1as; PI; KRAS; DLK1-DIO3 microRNA cluster; HUVECs
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2016.12.030

The cluster of microRNAs (miRNAs) in the DLK1-DIO3 genomic imprinted region contains several miRNAs that have a significant regulatory role in tumor proliferation and invasion. One of these miRNAs is miR-134-3p, and its expression changes significantly in human ovarian cancer stem cells (OCSCs) and in CD44−/CD133− ovarian cancer. The results of a luciferase assay showed that miR-134-3p silenced RAB27A by binding to the 3′-UTR of RAB27A mRNA. Overexpression of miR-134-3p in human OCSCs can not only inhibit the expression of RAB27A but also can effectively downregulate the expression of some tumor proliferation and invasion genes. Overexpression of miR-134-3p can not only inhibit the in vitro proliferation and cell cycle progression of human OCSCs but also can decrease the tumorigenicity in nude mice. •DLK1-DIO3 microRNA cluster has different expression between OCSCs and OCCs (CD44−/CD133−).•Low miR-134-3p in OCSCs correlates with RAB27a overexpression.•We confirmed RAB27a mRNA a novel target of miR-134-3p.•Overexpression for miR-134-3p inhibits OCSCs in vitro.•MiR-134-3p transfection decreases OCSCs xenograft tumor growth.