Temperature Effect on Protein Synthesis in a Heat-Synchronized Protozoan Treated with Actinomycin D

Protein synthesis was studied in the ciliated protozoan Tetrahymena pyriformis GL after actinomycin D was added to the culture medium. When the temperature rose above that optimum for growth, there were significant reductions in protein synthesis. Lipid biosynthesis under the same conditions was sli...

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Bibliographic Details
Published inScience (American Association for the Advancement of Science) Vol. 156; no. 3781; pp. 1504 - 1505
Main Authors Byfield, John E., Scherbaum, Otto H.
Format Journal Article
LanguageEnglish
Published United States American Association for the Advancement of Science 16.06.1967
Subjects
Acetates
Actinomycin
Amino Acids - metabolism
Antibiotics
Carbon Isotopes
Dactinomycin - pharmacology
Depression, Chemical
Glycerol - metabolism
Lipids
Lipids - biosynthesis
Messenger RNA
Molecules
Protein Biosynthesis
Protein synthesis
RNA
RNA stability
RNA, Messenger - metabolism
Temperature dependence
Tetrahymena - drug effects
Tetrahymena - metabolism
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Summary:Protein synthesis was studied in the ciliated protozoan Tetrahymena pyriformis GL after actinomycin D was added to the culture medium. When the temperature rose above that optimum for growth, there were significant reductions in protein synthesis. Lipid biosynthesis under the same conditions was slightly stimulated, an indication that the effect was not due to an underlying reduction in energy sources. The phenomenon appears to be unique to the protein synthesizing system. Correlation with previous data suggests that it is due to nontranslational destruction of template RNA.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0036-8075
1095-9203
DOI:10.1126/science.156.3781.1504