Protective effects of curcumin in APPswe transfected SH-SY5Y cells

• Published in: Neural regeneration research Vol. 7; no. 6; pp. 405 - 412
• Main Authors: Yin, Wenke, Zhang, Xiong, Li, Yu
• Format: Journal Article
• Language: English
• Published: India Medknow Publications & Media Pvt. Ltd 25.02.2012; Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China; Department of Pathology, North Sichuan Medical College, Nanchong 637000, Sichuan Province, China%Department of Pathology, Chongqing Medical University, Chongqing 400016, China; Medknow Publications & Media Pvt Ltd
• Subjects: Cell cycle; Enzymes; Kinases; Neuroblastoma; Neurosciences; Oxidative stress; Plasmids; Proteins; Reactive oxygen species; Research and Report: Traditional Chinese Medicine and Neuroregeneration; SH-SY5Y细胞; Signal transduction; Studies; 保护作用; 姜黄素; 神经母细胞瘤; 血红素加氧酶; 血红素氧合酶; 质粒转染; 阿尔茨海默氏病; United States > US; China; phosphatidylinositol 3-kinase signaling pathway; heme oxygenase-2; neural regeneration; heme oxygenase-1; Alzheimer's disease; curcumin
• ISSN: 1673-5374; 1876-7958
• DOI: 10.3969/j.issn.1673-5374.2012.06.001

The APPswe plasmid was transfected into the neuroblastoma cell line SH-SY5Y to establish a cell model of Alzheimer＇s disease. Graded concentration and time course experiments demonstrate that curcumin significantly upregulates phosphatidylinositol 3-kinase （PI3K）, Akt, nuclear factor E2-related factor-2 （Nrf2）, heme oxygenase 1 and ferritin expression, and that it significantly downregulates heme oxygenase 2, reactive oxygen species and amyloid-beta 40/42 expression. These effects of curcumin on PI3K, Akt and Nrf2 were blocked by LY294002 （PI3k inhibitor） and NF-E2-related factor-2 siRNA. The results indicate that the cytoprotection conferred by curcumin on APPswe transfected SH-SY5Y cells is mediated by its ability to regulate the balance between heme oxygenase 1 and 2 via the PI3K/Akt/Nrf2 intracellular signaling pathway.