Chemiluminescence-linked immunoassay

Covalent complexes of a chemiluminescent compound (a luminol derivative), protein and a steroidal hapten have been used as a labelled antigen in a way analogous to the radioactive label in radioimmunoassay. The antibody-bound complex emits light when treated with hydrogen peroxide and copper acetate...

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Bibliographic Details
Published inJournal of immunological methods Vol. 21; no. 1-2; p. 179
Main Authors Pratt, J J, Woldring, M G, Villerius, L
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1978
Subjects
Animals
Binding Sites, Antibody
Immunoassay
Luminescent Measurements
Rabbits
Testosterone
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Summary:Covalent complexes of a chemiluminescent compound (a luminol derivative), protein and a steroidal hapten have been used as a labelled antigen in a way analogous to the radioactive label in radioimmunoassay. The antibody-bound complex emits light when treated with hydrogen peroxide and copper acetate at high pH. The chemiluminescent complex bound to antibody can be displaced by free steroid to produce a standard curve in a way analogous to radioimmunoassay.
ISSN:0022-1759
DOI:10.1016/0022-1759(78)90234-X