Effect of 3,4-dihydroxyacetophenone on endothelial dysfunction in streptozotocin-induced rats with type 2 diabetes

• Published in: Journal of cardiovascular pharmacology Vol. 65; no. 1; p. 22
• Main Authors: Liu, Caiyan, Sun, Jie, Xue, Feng, Yi, Yanchun, Han, Aiqiang
• Format: Journal Article
• Language: English
• Published: United States 01.01.2015
• Subjects: Acetophenones - pharmacology; Animals; Aorta, Thoracic - drug effects; Aorta, Thoracic - pathology; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - physiopathology; Diabetes Mellitus, Type 2 - drug therapy; Diabetes Mellitus, Type 2 - physiopathology; Endothelium, Vascular - drug effects; Endothelium, Vascular - pathology; Male; NF-kappa B - metabolism; Nitric Oxide - metabolism; Nitric Oxide Synthase Type III - metabolism; Oxidative Stress - drug effects; Rats; Rats, Sprague-Dawley; Streptozocin; Superoxides - metabolism; Vasodilation - drug effects
• ISSN: 1533-4023
• DOI: 10.1097/FJC.0000000000000158

This study investigated whether 3,4-Dihydroxyacetophenone (DHAP) could improve endothelial function in streptozotocin-induced type 2 diabetic rats. Sprague-Dawley rats were randomly divided into control, diabetic, and diabetic DHAP-treated animals. After treatment with DHAP for 8 weeks, endothelial function was determined by measuring endothelium-dependent vasodilatation (EDV) of the thoracic aorta. Endothelial nitric oxide synthase (eNOS) activity and nitric oxide (NO) production in endothelial cells and nuclear transcription factor kappa B (NF-κB) expression and superoxide anion production in the aorta were determined. DHAP treatment reduced serum levels of triglycerides, cholesterol, malondialdehyde, and tumor necrosis factor α, and enhanced serum adiponectin levels. Endothelium-dependent vasodilatation was significantly attenuated in rats with diabetes and increased significantly after DHAP treatment. NO levels and eNOS activity in endothelial cells were significantly reduced, and NF-κB activation and superoxide production increased in rats with diabetes compared with the control group. DHAP treatment enhanced NO levels and eNOS activity and decreased NF-κB activation and superoxide production. These findings suggest that DHAP could improve endothelial function in streptozotocin-induced type 2 diabetic rats. The mechanism may be related to the enhancement of eNOS activity and NO production by reducing plasma lipid levels, oxidative stress, and inflammatory activity.