The Diversity and Distribution of Viruses Associated with Culex annulirostris Mosquitoes from the Kimberley Region of Western Australia

Metagenomics revealed an impressive breadth of previously unrecognized viruses. Here, we report the virome of the Skuse mosquito, an important vector of pathogenic arboviruses in Australia. Mosquitoes were collected from three sites in the Kimberley region of Western Australia. Unbiased high-through...

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Published inViruses Vol. 12; no. 7; p. 717
Main Authors Williams, Simon H, Levy, Avram, Yates, Rachel A, Somaweera, Nilusha, Neville, Peter J, Nicholson, Jay, Lindsay, Michael D A, Mackenzie, John S, Jain, Komal, Imrie, Allison, Smith, David W, Lipkin, W Ian
Format Journal Article
LanguageEnglish
Published Switzerland MDPI 02.07.2020
MDPI AG
Subjects
Animals
Culex - virology
Culex annulirostris
High-Throughput Nucleotide Sequencing
Metagenomics
Mosquito Vectors - virology
Virome
Viruses - classification
Viruses - isolation & purification
Western Australia
Western Australia
virome
Culex annulirostris
Western Australia
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Summary:Metagenomics revealed an impressive breadth of previously unrecognized viruses. Here, we report the virome of the Skuse mosquito, an important vector of pathogenic arboviruses in Australia. Mosquitoes were collected from three sites in the Kimberley region of Western Australia. Unbiased high-throughput sequencing (HTS) revealed the presence of 16 novel viral sequences that share less than 90% identity with known viruses. None were closely related to pathogenic arboviruses. Viruses were distributed unevenly across sites, indicating a heterogeneous virome. Polymerase chain reaction assays confirmed HTS data and identified marked variation between the virus prevalence identified at each site.
ISSN:1999-4915
1999-4915
DOI:10.3390/v12070717