Microsatellite markers for the Chinese herbaceous peony Paeonia lactiflora (Paeoniaceae)

• Premise of the study: Microsatellite (simple sequence repeat, SSR) primers were developed for the Chinese peony, P. lactiflora, to investigate the diversity within Chinese peony germplasm resources. • Methods and Results: Using an SSR‐enriched genomic library, a set of 10 unique polymorphic genomi...

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Published inAmerican journal of botany Vol. 98; no. 2; pp. e16 - e18
Main Authors Li, Lin, Cheng, Fang‐yun, Zhang, Qi‐xiang
Format Journal Article
LanguageEnglish
Published United States Botanical Society of America 01.02.2011
Botanical Society of America, Inc
Subjects
Alleles
Botany
DNA Primers
DNA, Plant
enriched library
Gene loci
Genetic diversity
Genetic Loci
Genetic markers
Genetic research
Genome, Plant
Genomics
Genotype
Herbs
microsatellite
Microsatellite Repeats
Paeonia - genetics
Paeonia lactiflora
Polymorphism
Polymorphism, Genetic
primer
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Summary:• Premise of the study: Microsatellite (simple sequence repeat, SSR) primers were developed for the Chinese peony, P. lactiflora, to investigate the diversity within Chinese peony germplasm resources. • Methods and Results: Using an SSR‐enriched genomic library, a set of 10 unique polymorphic genomic SSRs was developed and characterized. The primers amplified 61 alleles in all 10 loci, including di‐, tri‐, and tetranucleotide repeats. The primers were also effective for P. veitchii and P. obovata. • Conclusions: The new primers will be useful for genetic research of the Chinese peony and extend the repertoire of SSR markers available to study the herbaceous taxa in Paeonia.
Bibliography:The authors thank Ms. Beth Hazen at Willows End for language improvement. This project is supported by the National Science and Technology Support Program of China (2006BAD01A1801), Key Project for Forestry Science Research (2006‐40), and Co‐constructive Project of Beijing Education Committee (2009).
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SourceType-Scholarly Journals-1
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ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1000410