New Composite Modified Carbon Microfibers for Sensitive and Selective Determination of Physiologically Relevant Concentrations of Nitric Oxide in Solution

• Published in: Electroanalysis (New York, N.Y.) Vol. 11; no. 12; pp. 845 - 850
• Main Authors: Pontié, Maxime, Bedioui, Fethi, Devynck, Jacques
• Format: Journal Article
• Language: English
• Published: Weinheim VCH Verlagsgesellschaft mbH 01.08.1999
• Subjects: Electrochemical microsensor; Interference; Nafion; Nickel porphyrin; Nitric oxide; Phenylene derivatives; Selectivity; Sensitivity
• ISSN: 1040-0397; 1521-4109
• DOI: 10.1002/(SICI)1521-4109(199908)11:12<845::AID-ELAN845>3.0.CO;2-D

In this study, we have prepared nitric oxide (NO) microsensors using four combinations of nickel porphyrin (NiP), o‐phenylenediamine (o‐PD), m‐phenylenediamine (m‐PD), resorcinol and Nafion based layers to modify the surface of 8 μm diameter carbon fiber electrodes. We have compared the performances of the obtained composite microsensors (NiP/Nafion, Nafion/o‐PD, Nafion/(m‐PD+resorcinol), and NiP/Nafion/o‐PD, respectively) in our home operating conditions. Our results indicate that Nafion/o‐PD and Nafion/(m‐PD+resorcinol) microsensors are capable of detecting NO very selectively against nitrites, ascorbate, L‐arginine, serotonine and xanthine. But these microsensors have a poorer sensitivity to NO than the NiP/Nafion/o‐PD microsensor. If selectivity is not a concern, NiP/Nafion, Nafion/o‐PD and NiP/Nafion/o‐PD microsensors are the most sensitive to NO concentrations as low as 40 nmol L–1.