Translocation detection in lymphoma diagnosis by split-signal FISH: a standardised approach

Lymphomas originating from the lymphatic system comprise about 30 entities classified according to the World Health Organization (WHO). The histopathological diagnosis is generally considered difficult and prone to mistakes. Since non-random chromosomal translocations are specifically involved in di...

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Published inJournal of hematopathology Vol. 1; no. 2; pp. 119 - 126
Main Authors van Rijk, Anke, Mason, David, Jones, Margaret, Cabeçadas, José, Crespo, Mateus, Cigudosa, Juan Cruz, Garcia, Juan Fernando, Leoncini, Lorenzo, Cocco, Mario, Hansmann, Martin-Leo, Mottok, Anja, Copie Bergman, Christiane, Baia, Maryse, Anagnostou, Dimitra, Pouliou, Evi, Hamilton Dutoit, Stephen, Hjøllund Christiansen, Mette, Svenstrup Poulsen, Tim, Hauge Matthiesen, Steen, van Dongen, Jacques, van Krieken, J. Han
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer-Verlag 01.09.2008
Subjects
Hematology
Medicine
Medicine & Public Health
Original
Original Article
Pathology
Validation
Chromosomal aberration
Split-signal FISH
Lymphoma
Classification
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Summary:Lymphomas originating from the lymphatic system comprise about 30 entities classified according to the World Health Organization (WHO). The histopathological diagnosis is generally considered difficult and prone to mistakes. Since non-random chromosomal translocations are specifically involved in different lymphoma entities, their detection will be increasingly important. Hence, a split-signal fluorescence in situ hybridisation (FISH) procedure would be helpful in discriminating the most difficult classifications. The Euro-FISH programme, a concerted action of nine European laboratories, has validated a robust, standardised protocol to improve the diagnostic approach on lymphoma entities. Therefore, 16 fluorescent probes and 10 WHO entities, supplemented with reactive cases, were selected. The results of the Euro-FISH programme show that all probes were correctly cytogenetically located, that the standardised protocol is robust, resulting in reliable results in approximately 90% of cases, and that the procedure could be implemented in every laboratory, bringing the relatively easy interpretation of split-signal probes within the reach of many pathology laboratories.
ISSN:1865-5785
1868-9256
1865-5785
DOI:10.1007/s12308-008-0017-5