A set of microsatellite markers for Arrabidaea chica (Bignoniaceae), a medicinal liana from the Neotropics

• Premise of the study: Microsatellite markers were developed, optimized, and characterized for Arrabidaea chica (Humb. & Bonpl.) Verl. (Bignoniaceae), a Neotropical liana extensively used in folk medicine. The aim of this study was to develop molecular tools to investigate the genetic structure...

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Published inAmerican journal of botany Vol. 97; no. 7; pp. e63 - e64
Main Authors Figueira, Glyn M., Ramelo, Pamela R., Ogasawara, Daniela C., Montanari, Ilio, Zucchi, Maria I., M.Cavallari, Marcelo, Foglio, Mary A.
Format Journal Article
LanguageEnglish
Published United States Botanical Society of America 01.07.2010
Botanical Society of America, Inc
Subjects
Arrabidaea chica
Bignoniaceae
Botany
Flowers & plants
Genetic diversity
Genetics
microsatellite markers
Molecular structure
Polymorphism
SSR
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Summary:• Premise of the study: Microsatellite markers were developed, optimized, and characterized for Arrabidaea chica (Humb. & Bonpl.) Verl. (Bignoniaceae), a Neotropical liana extensively used in folk medicine. The aim of this study was to develop molecular tools to investigate the genetic structure and diversity of natural populations and germplasm collections of this species. • Methods and Results: Eight highly polymorphic microsatellite markers revealed a multibanded pattern, suggesting that the species is polyploid. The total number of bands per locus ranged from 9 to 17, revealing high levels of polymorphism. • Conclusions: The high level of polymorphism detected with these markers indicates their utility in devising conservation strategies and rational exploitation of A. chica.
Bibliography:This research was supported by Fundação de Amparo á Pesquisa do Estado de São Paulo (FAPESP).
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ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1000145