Subcutaneous tissue reaction and gene expression of inflammatory markers after Biodentine and MTA implantation

• Published in: Brazilian dental journal Vol. 33; no. 1; pp. 41 - 56
• Main Authors: Silva, Raquel Assed Bezerra, Gaton-Hernandez, Patrícia, Pucinelli, Carolina Maschietto, Silva, Francisco Wanderley Garcia de Paula E, Lucisano, Marília Pacífico, Consolaro, Alberto, Sá, Rafaela Cardoso de, Araujo, Lisa Danielly Curcino, Sousa-Neto, Manoel Damião, Silva, Léa Assed Bezerra
• Format: Journal Article
• Language: English
• Published: Brazil Fundação Odontológica de Ribeirão Preto 01.01.2022
• Subjects: Aluminum Compounds - pharmacology; Animals; Calcium Compounds - pharmacology; Drug Combinations; Gene Expression; Mice; Oxides - pharmacology; Root Canal Filling Materials - pharmacology; Silicates - pharmacology; Subcutaneous Tissue
• ISSN: 0103-6440; 1806-4760
• DOI: 10.1590/0103-6440202203562

The aim of this study was to evaluate the subcutaneous connective tissue response of isogenic mice exposed to tricalcium silicate (Biodentine) and aggregated mineral trioxide (ProRoot MTA). A total of 120 mice were divided into 4 groups in 3 different experimental periods (7, 21 and 63 days): Biodentine; Pro-Root MTA; zinc oxide-eugenol and; Negative control - Sham. After the experimental periods microscopic descriptive, semi-quantitative and quantitative analysis of the inflammatory process were analyzed on H&E sections and evaluation of the gene expression of Il10, Infg, Il6, Il1r1 and Tnf (qRT-PCR) were performed. The data obtained were analyzed using the chi-square test and two-way analysis of variance (ANOVA) followed by the Bonferroni post-test (5% significance level). Results: In the microscopic analysis, a slight inflammatory infiltrate was observed, with a predominance of sparse macrophages and polymorphonuclear cells, slight tissue fibrosis, regular fibrous capsule and with dystrophic calcifications, in all groups that received the materials (Biodentine and Pro-Root MTA). In parallel, all materials modulated the gene expression of the different cytokines and receptors evaluated. Conclusion: Pro-Root MTA and Biodentine showed a tissue compatibility, mediated inflammation, with increased fibrous tissue and production of pro- and anti-inflammatory cytokines.