Easy Synthesis of Doped Graphitic Carbon Nitride Nanosheets as New Material for Enhanced DNA Extraction from Vegetal Tissues Using a Simple and Fast Protocol

Conventional and commercially available DNA extraction methods have several limitations regarding, for instance, contamination, and complex and slow precipitation and recovery processes. Herein, we report the synthesis of oxygen and phosphorus-doped Graphitic carbon nitride structures (g-POCN), via...

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Bibliographic Details
Published inC (Basel) Vol. 8; no. 4; p. 68
Main Authors Martínez-Cartagena, Manuel Eduardo, Bernal-Martínez, Juan, Banda-Villanueva, Arnulfo, Lechuga-Islas, Víctor D., Córdova, Teresa, Magaña, Ilse, Torres-Lubián, José Román, Fernández-Tavizón, Salvador, Romero-García, Jorge, Rodríguez-Hernández, Ana Margarita, Díaz-de-León, Ramón
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.12.2022
Subjects
Biocompatibility
Biosensors
Carbon
Carbon nitride
Chemicals
Deoxyribonucleic acid
DNA
DNA extraction
DNA purification
Graphene
graphitic carbon nitride
Laboratories
Methods
Nanomaterials
Nanosheets
NMR
Nuclear magnetic resonance
PCR
Phosphorus
Polymerase chain reaction
Solvents
solvothermal synthesis
Spectrum analysis
Synthesis
Zinc
St Louis Missouri
United States > US
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Summary:Conventional and commercially available DNA extraction methods have several limitations regarding, for instance, contamination, and complex and slow precipitation and recovery processes. Herein, we report the synthesis of oxygen and phosphorus-doped Graphitic carbon nitride structures (g-POCN), via a novel Zinc-catalyzed one-pot solvothermal approach, and its application in the extraction of genomic DNA (gDNA) from a vegetal matrix (P. argentatum). Experimental and molecular modeling analyses demonstrate the high affinity of gDNA with g-POCN, which provided highly efficient gDNA extraction processes, with extraction yield, as well as integrity and quality of the extracted gDNA, comparable or superior to a commercial extraction kit and isopropanol extraction. Moreover, under suitable elution conditions, this method allows the easy removal of high concentrations of gDNA from g-POCN, rendering this method as a low-cost, simple, and fast approach for the extraction of even small amounts of gDNA. Remarkably, the extracted gDNA shows no degradation, and no inhibition of the polymerase chain reaction. Therefore, g-POCN represents a promising material for the highly efficient, cost-effective, and biocompatible extraction of DNA, which could stimulate research focused on broad DNA sources, e.g., RNA extraction, plasmids, ssDNA, etc.
ISSN:2311-5629
2311-5629
DOI:10.3390/c8040068