Experimental reproduction of hypoglycemia-spiking mortality syndrome in broiler chickens with the use of homogenized brains containing arenaviruslike particles

Severe hypoglycemia-spiking mortality syndrome was experimentally reproduced in broiler chicks. Inoculum was homogenized brains from 28-day-old commercial broiler chicks with central nervous system signs (50% [v/v] in phosphate-buffered saline with 2% fetal calf serum). Oral inoculations of 1.2 ml o...

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Bibliographic Details
Published inAvian diseases Vol. 41; no. 2; p. 442
Main Authors Davis, J.F. (Georgia Poultry Laboratory, Oakwood.), De La Torre, J.C, Castro, A.E, Connolly, B.M, Doman, J.T, Dunn, P.A
Format Journal Article
LanguageEnglish
Published United States 01.04.1997
Subjects
Animals
ARENAVIRIDAE
Arenaviridae Infections - pathology
Arenaviridae Infections - physiopathology
Arenaviridae Infections - veterinary
Arenavirus - isolation & purification
Arenavirus - pathogenicity
AVES DE CORRAL
Brain - pathology
Brain - ultrastructure
Brain - virology
Brain Diseases - pathology
Brain Diseases - veterinary
Brain Diseases - virology
CEREBRO
Chickens
ENCEPHALE
ENFERMEDADES DE LOS ANIMALES
HIPOGLICEMIA
Hypoglycemia - pathology
Hypoglycemia - physiopathology
Hypoglycemia - veterinary
HYPOGLYCEMIE
INFECCION EXPERIMENTAL
INFECTION EXPERIMENTALE
MALADIE DES ANIMAUX
Microscopy, Electron
POLLO DE ENGORDE
POULET DE CHAIR
Poultry Diseases
Reference Values
Syndrome
VOLAILLE
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Summary:Severe hypoglycemia-spiking mortality syndrome was experimentally reproduced in broiler chicks. Inoculum was homogenized brains from 28-day-old commercial broiler chicks with central nervous system signs (50% [v/v] in phosphate-buffered saline with 2% fetal calf serum). Oral inoculations of 1.2 ml of the homogenate were given at 1 day of age to broiler chicks (n = 15). Fourteen days later, chicks were fasted and stressed with a 2-sec cool water spray. Six chicks (40%) developed clinical signs of spiking mortality syndrome and were severely hypoglycemic. Uninoculated control chicks (n = 15) from the same hatch, also fasted and stressed simultaneously, were unaffected. Examination of a banded fraction produced from the inoculum with the use of transmission electron microscopy with negative staining revealed viruslike particles indistinguishable from arenavirus particles stained and examined simultaneously. Avian encephalomyelitis virus was isolated by one of three laboratories attempting virus isolation with the use of embryonating chicken eggs
Bibliography:L73
9747659
ISSN:0005-2086
1938-4351
DOI:10.2307/1592202