A rapid radioimmunoassay for human α-fetoprotein

Our previous double antibody radioimmunoassay for human AFP was run at 4°C and required 48 h for completion. A method is described which permits the assay to be performed within 5 h due to an increased incubation temperature and the use of polyethylene glycol to precipitate the antigen-antibody comp...

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Bibliographic Details
Published inClinica chimica acta Vol. 86; no. 1; pp. 67 - 72
Main Authors Blank-Liss, W.E., Lai, P.C.W., Hay, D.M., Lorscheider, F.L.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 16.05.1978
Subjects
alpha-Fetoproteins - analysis
Animals
Goats - immunology
Humans
Methods
Polyethylene Glycols
Rabbits - immunology
Radioimmunoassay - methods
Temperature
Time Factors
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Summary:Our previous double antibody radioimmunoassay for human AFP was run at 4°C and required 48 h for completion. A method is described which permits the assay to be performed within 5 h due to an increased incubation temperature and the use of polyethylene glycol to precipitate the antigen-antibody complex. The modified radioimmunoassay was standardized against an international reference preparation of human AFP. Characteristics of this assay are discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(78)90459-X