A rapid radioimmunoassay for human α-fetoprotein
Our previous double antibody radioimmunoassay for human AFP was run at 4°C and required 48 h for completion. A method is described which permits the assay to be performed within 5 h due to an increased incubation temperature and the use of polyethylene glycol to precipitate the antigen-antibody comp...
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Published in | Clinica chimica acta Vol. 86; no. 1; pp. 67 - 72 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
16.05.1978
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Subjects | |
Online Access | Get full text |
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Summary: | Our previous double antibody radioimmunoassay for human AFP was run at 4°C and required 48 h for completion. A method is described which permits the assay to be performed within 5 h due to an increased incubation temperature and the use of polyethylene glycol to precipitate the antigen-antibody complex. The modified radioimmunoassay was standardized against an international reference preparation of human AFP. Characteristics of this assay are discussed. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0009-8981 1873-3492 |
DOI: | 10.1016/0009-8981(78)90459-X |