Location of single neurons containing HRP for electron microscopy

A technique is described for finding single neurons containing transported horseradish peroxidase (HRP) for study in the electron microscope. Tissue chopper sections which have been reacted to demonstrate HRP are cleared in glycerol and examined with darkfield light microscopy. The granular reaction...

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Bibliographic Details
Published inBrain research bulletin Vol. 2; no. 6; p. 495
Main Authors Price, P G, Fisher, A W
Format Journal Article
LanguageEnglish
Published United States 01.11.1977
Subjects
Animals
Axonal Transport
Horseradish Peroxidase - analysis
Hypothalamus - ultrastructure
Male
Methods
Neurons - ultrastructure
Peroxidases - analysis
Pituitary Gland, Posterior - ultrastructure
Rats
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Summary:A technique is described for finding single neurons containing transported horseradish peroxidase (HRP) for study in the electron microscope. Tissue chopper sections which have been reacted to demonstrate HRP are cleared in glycerol and examined with darkfield light microscopy. The granular reaction product is clearly seen, enabling accurate drawings to be made for later dissection for ultramicrotomy. The neuron sought can then be found within a few microns of the cutting face of the block. While some distortion does occur, most of the cellular morphology remains intact, enabling recognition of specific organelles such as neurosecretory granules in cells which have transported HRP from the neurohypophysis.
ISSN:0361-9230
DOI:10.1016/0361-9230(77)90059-4