Effect of Dietary Betaine on Oxidative Stress and Immune Response in Postpartum Lactating Murrah Buffaloes (Bubalus bubalis) during Hot-Humid Season

• Published in: Pakistan journal of zoology Vol. 55; no. 6; p. 2935
• Main Authors: Shakkarpude, Jyotsana, Mishra, Aditya, Caesar, Deepika D, Sharma, R.K, Jain, Anand Kumar, Mandal, Sanju, Vandre, Rajesh Kumar, Yadav, Danveer S, Ahirwar, Bhavna, Solanki, C.P
• Format: Journal Article
• Language: English
• Published: Lahore Knowledge Bylanes 31.12.2023; AsiaNet Pakistan (Pvt) Ltd
• Subjects: Albumin; Albumins; Animal lactation; Animals; Antioxidants; Appetite; Betaine; Biochemistry; Bubalus bubalis; Buffalo; Cattle; Control; Diet; Energy balance; Experiments; Feeds; Food and nutrition; Free radicals; Globulins; Health aspects; Heat; Heat shock proteins; Hsp70 protein; Humidity; Immune response; Immune system; Lactation; Livestock; Oxidative stress; Physiological aspects; Physiology; Plasma; Postpartum; Superoxide dismutase; Water buffalo; India; Los Angeles California; United States > US
• ISSN: 0030-9923; 0030-9923
• DOI: 10.17582/journal.pjz/20211208161255

ABSTRACT Excessive heat and humidity causes reduction in appetite of postpartum buffaloes which directly leads negative energy balance (NEBAL) and increases oxidative stress in animals. Betaine is a growth promoting nutritional additive used in livestock. Betaine contains three methyl groups and thus acts as a methyl donor for metabolic reactions. Betaine acts as molecular chaperone, as it repairs heat shock proteins. The present study was aimed to investigate the effect of dietary betaine on heat and metabolic stress during lactation in Murrah buffaloes (Bubalus bubalis). For this purpose, a total of 18 postpartum Murrah buffaloes were randomly divided into control, low betaine and high betaine groups supplemented with betaine @ 50 g/animal/day and 100 g/animal/day respectively from day 5 postpartum and was continued up to 4 months postpartum. 5ml of blood sample was collected from animal for analysis of superoxide dismutase, total antioxidant capacity, HSP70, albumin/globulin ratio and immunoglobin G. The overall mean values of plasma superoxide dismutase, total antioxidant capacity and immunoglobin G of high betaine group was numerically higher than other groups which differed (p<0.05) significantly between groups. The overall mean value of plasma HSP70 of high betaine group was minimum followed by low betaine group and control group had maximum mean value which differed (p<0.05) significantly between groups. The overall mean values of albumin/globulin ratio of control group were higher than the betaine groups which differed (p<0.05) significantly between groups.