A biosynthesis chamber system for the production of isotopically labeled tobacco

This paper describes the design, fabrication, and operation of a special plant growth chamber system utilized in the biosynthetic production of carbon-14 labeled tobacco plant material. The controlled environmental factors of the system were relative humidity, temperature, carbon dioxide, nutrients...

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Bibliographic Details
Published inThe International journal of applied radiation and isotopes Vol. 26; no. 12; pp. 753 - 757
Main Authors Bass, R.T., Jenkins, R.W., Newell, G.C., Osdene, T.S.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 1975
Subjects
field crops
plant biochemistry
plant physiology
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Summary:This paper describes the design, fabrication, and operation of a special plant growth chamber system utilized in the biosynthetic production of carbon-14 labeled tobacco plant material. The controlled environmental factors of the system were relative humidity, temperature, carbon dioxide, nutrients and auxiliary lighting. Four plants of Nicotiana tabacum L. cv. Coker 254 were grown for five weeks in the 14CO 2 atmosphere of the growth chamber. The four-plant harvest produced a total dry weight yield of 420 g of uniformly labeled 14C-tobacco plant material with an average specific activity of 614 nCi/mg of carbon.
ISSN:0020-708X
DOI:10.1016/0020-708X(75)90134-9